PMID:3536855
| Citation |
Surin, BP, Dixon, NE and Rosenberg, H (1986) Purification of the phoU protein, a negative regulator of the pho regulon of Escherichia coli K-12. J. Bacteriol. 168:631-5 |
|---|---|
| Abstract |
Thermally induced transcription of the phoU gene under control of the major leftward promoter, pL, of phage lambda resulted in production of the PhoU protein to compose approximately 5% of the total cell protein. The PhoU protein was present in the cytoplasm in the form of an aggregate. The amino acid composition and N-terminal amino acid sequence of the purified protein confirmed the reading frame established earlier for the phoU gene. |
| Links | |
| Keywords |
Amino Acid Sequence; Amino Acids/analysis; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Biological Transport; Escherichia coli/analysis; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Bacterial; Genes, Regulator; Membrane Transport Proteins; Phosphates/metabolism; Promoter Regions, Genetic; Transcription Factors |
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