PMID:3536662
| Citation |
Buoncristiani, MR, Howard, PK and Otsuka, AJ (1986) DNA-binding and enzymatic domains of the bifunctional biotin operon repressor (BirA) of Escherichia coli. Gene 44:255-61 |
|---|---|
| Abstract |
The negative regulation of the biotin biosynthetic (bio) operon in Escherichia coli is mediated by the bifunctional birA gene product, which serves as the bio repressor and biotin-activating enzyme. Nucleotide sequence analysis of 18 mutations in the birA gene was employed to study the DNA-binding and enzymatic functions of the BirA protein. The results indicate that a predicted helix-turn-helix structure, from amino acid (aa) positions 18 to 39 within the 321-aa BirA protein, may be responsible for sequence-specific DNA binding, whereas the temperature-sensitive mutations affecting biotin activation are found in two regions from aa positions 83-119 and 189-235. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Biotin/pharmacology; DNA, Bacterial/metabolism; Escherichia coli/drug effects; Escherichia coli/genetics; Genes; Genes, Bacterial; Genetic Complementation Test; Mutation; Operon; Repressor Proteins/genetics; Repressor Proteins/metabolism; Transcription Factors/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.