PMID:3511033
| Citation |
Andrews, JC, Blevins, TC and Short, SA (1986) Regulation of peptide transport in Escherichia coli: induction of the trp-linked operon encoding the oligopeptide permease. J. Bacteriol. 165:428-33 |
|---|---|
| Abstract |
Growth of Escherichia coli in medium containing leucine results in increased entry of exogenously supplied tripeptides into the bacterial cell. This leucine-mediated elevation of peptide transport required expression of the trp-linked opp operon and was accompanied by increased sensitivity to toxic tripeptides, by an enhanced capacity to utilize nutritional peptides, and by an increase in both the velocity and apparent steady-state level of L-[U-14C]alanyl-L-alanyl-L-alanine accumulation for E. coli grown in leucine-containing medium relative to these parameters of peptide transport measured with bacteria grown in media lacking leucine. Direct measurement of opp operon expression by pulse-labeling experiments demonstrated that growth of E. coli in the presence of leucine resulted in increased synthesis of the oppA-encoded periplasmic binding protein. |
| Links | |
| Keywords |
Biological Transport, Active; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation/drug effects; Genetic Linkage; Leucine/pharmacology; Membrane Transport Proteins/genetics; Molecular Weight; Oligopeptides/metabolism; Operon; Tryptophan/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.