PMID:3510672

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Citation

Prieto-Santos, MI, Martín-Checa, J, Balaña-Fouce, R and Garrido-Pertierra, A (1986) A pathway for putrescine catabolism in Escherichia coli. Biochim. Biophys. Acta 880:242-4

Abstract

Escherichia coli mutants able to grow in putrescine have been isolated from gamma-aminobutyrate mutants. These mutants show putrescine-alpha-ketoglutarate transaminase and gamma-aminobutyraldehyde dehydrogenase activities. Both enzymes have been characterized, the first of them showing an apparent Km for putrescine of 22.5 microM and the second an apparent Km of 37 microM for NAD and 18 microM for delta-1-pyrroline; the optimum pH values were 7.2 and 5.4, respectively, for the two enzymes.

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Keywords

Aldehyde Oxidoreductases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Mutation; Putrescine/metabolism; Transaminases/metabolism

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