PMID:3510127

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Citation

Kornberg, H (1986) The roles of HPr and FPr in the utilization of fructose by Escherichia coli. FEBS Lett. 194:12-5

Abstract

A mutant impaired in FPr activity was isolated. The altered gene (fpr), which was located near min. 44 on the E. coli genome, was transferred by phage-mediated transduction to appropriate recipients that lack HPr (ptsH), or Enzyme IIman (ptsM), or neither. The rates of growth on fructose of such transductants indicate that phosphate from PEP is transferred predominantly via FPr to fructose that enters the cells by Enzyme IIfru, but that HPr can play a role in transferring phosphate to fructose taken up via Enzyme IIman.

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FEBS Letters

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport, Active; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chromosomes, Bacterial; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Fructose/metabolism; Genes; Genes, Bacterial; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Phosphorylation

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