PMID:350869
| Citation |
Dev, IK and Harvey, RJ (1978) N10-Formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in Escherichia coli. J. Biol. Chem. 253:4242-4 |
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| Abstract |
Glycinamide ribotide transformylase from Escherichia coli was obtained free of N5,N10-methenyltetrahydrofolate cyclohydrolase activity by DEAE-cellulose chromatography. In reaction mixtures containing this enzyme preparation in potassium maleate buffer, pH 7.2, no detectable interconversion of N5,N10-methenyltetrahydrofolate occurred. Upon addition of glycinamide ribotide, N-formylglycinamide ribotide was formed when N10-formyltetrahydrofolate was present; no formylation occurred in the presence of N5,N10-methenyltetrahydrofolate. A method for the synthesis and purification of glycinamide ribotide is presented. |
| Links | |
| Keywords |
Acyltransferases/isolation & purification; Escherichia coli/enzymology; Formates; Formyltetrahydrofolates; Glycine; Kinetics |
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