PMID:342527
| Citation |
Munson, RS Jr, Rasmussen, NS and Osborn, MJ (1978) Biosynthesis of lipid A. Enzymatic incorporation of 3-deoxy-D-mannooctulosonate into a precursor of lipid A in Salmonella typhimurium. J. Biol. Chem. 253:1503-11 |
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| Abstract |
The cell envelope fraction of Salmonella typhimurium contains an enzyme system which catalyzes transfer of 3-deoxyoctulosonate (KDO) from CMP-KDO to an incomplete, KDO-deficient precursor of lipid A. The enzyme system is firmly membrane-bound, but has been solubilized by treatment with nonionic detergent at alkaline pH and partially purified. Both the particulate and partially purified fractions catalyzed formation of a single reaction product containing 2 residues of KDO. Periodate oxidation of the purified product permitted tentative identification of the KDO disaccharide structure as KDO2-4KDO. |
| Links | |
| Keywords |
Cell Membrane/enzymology; Ketoses/metabolism; Kinetics; Lipid A/biosynthesis; Lipid A/isolation & purification; Lipopolysaccharides/biosynthesis; Salmonella typhimurium/metabolism; Sugar Acids/metabolism; Transferases/metabolism |
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