PMID:334745
| Citation |
O'Brien, WJ and Frerman, FE (1977) Evidence for a complex of three beta-oxidation enzymes in Escherichia coli: induction and localization. J. Bacteriol. 132:532-40 |
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| Abstract |
The enzymes for beta-oxidation of fatty acids in inducible and constitutive strains of Escherichia coli were assayed in soluble and membrane fractions of disrupted cells by using fatty acid and acyl-coenzyme A (CoA) substrates containing either 4 or 16 carbon atoms in the acyl moieties. Cell fractionation was monitored, using succinic dehydrogenase as a membrane marker and glucose 6-phosphate dehydrogenase as a soluble marker. Acyl-CoA synthetase activity was detected exclusively in the membrane fraction, whereas acyl-CoA dehydrogenase, 3-hydroxyacyl-CoA dehydrogenase, enoyl-CoA hydratase, and 3-ketoacyl-CoA thiolase activities that utilized both C4 and C16 acyl-CoA substrates were isolated from the soluble fraction. 3-Hydroxyacyl-CoA dehydrogenase, enoyl-CoA hydratase, and 3-ketoacyl-CoA thiolase activities assayed with both C4 and C16 acyl-CoA substrates co-chromatographed on gel filtration and ion-exchange columns and cosedimented in glycerol gradients. The data show that these three enzyme activities of the fad regulon can be isolated as a multienzyme complex. This complex dissociates in very dilute preparations; however, in those preparations where the three activities are separated, the fractionated species retain activity with both C4 and C16 acyl-CoA substrates. |
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| Keywords |
3-Hydroxyacyl CoA Dehydrogenases/biosynthesis; 3-Hydroxyacyl CoA Dehydrogenases/metabolism; Acetates/metabolism; Acetyl-CoA C-Acyltransferase/biosynthesis; Acetyl-CoA C-Acyltransferase/metabolism; Acyl Coenzyme A/metabolism; Acyl-CoA Dehydrogenases/biosynthesis; Acyl-CoA Dehydrogenases/metabolism; Acyltransferases/biosynthesis; Cell Membrane/enzymology; Coenzyme A Ligases/biosynthesis; Coenzyme A Ligases/metabolism; Cytoplasm/enzymology; Enoyl-CoA Hydratase/biosynthesis; Enoyl-CoA Hydratase/metabolism; Enzyme Induction; Escherichia coli/enzymology; Escherichia coli/metabolism; Fatty Acids/metabolism; Hydro-Lyases/biosynthesis; Multienzyme Complexes/biosynthesis; Multienzyme Complexes/metabolism; Oleic Acids/metabolism; Subcellular Fractions |
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