PMID:3323532
| Citation |
Loenen, WA, Daniel, AS, Braymer, HD and Murray, NE (1987) Organization and sequence of the hsd genes of Escherichia coli K-12. J. Mol. Biol. 198:159-70 |
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| Abstract |
The nucleotide sequence of the hsdR and M genes, together with that for hsdS comprises an 8400 base segment spanning the entire hsd region of Escherichia coli K-12. The three hsd genes are transcribed in the same direction, but from two promoters. hsdR and hsdM are separated by 492 base-pairs, whereas the termination codon of hsdM overlaps the initiation codon of hsdS. pres precedes hsdR, and our data indicate a transcription termination signal in the interval between hsdR and pmod, as expected if transcription of hsdM and S is dependent on pmod. Transcription from pres is not influenced by the products of the hsdM and S genes, and the mechanism whereby restriction is prevented when the hsd region is transferred to a modification-deficient cell remains to be elucidated. A segment of the predicted amino acid sequence of the M polypeptide shares homology with a variety of adenine methylases and may identify part of the active site for methylation of specific adenine residues. The R polypeptide shows homology with a variety of ATPases, and pronounced regions of alpha-helical structure are predicted, one of which is amphipathic. |
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| Keywords |
Amino Acid Sequence; Base Sequence; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli/genetics; Genes, Bacterial; Methyltransferases; Molecular Sequence Data; Protein Biosynthesis; Sequence Homology, Nucleic Acid; Site-Specific DNA-Methyltransferase (Adenine-Specific); Terminator Regions, Genetic; Transcription, Genetic |
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