PMID:3316180
| Citation |
Griggs, DW, Tharp, BB and Konisky, J (1987) Cloning and promoter identification of the iron-regulated cir gene of Escherichia coli. J. Bacteriol. 169:5343-52 |
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| Abstract |
The cir gene, which encodes the colicin I receptor protein and is regulated by both cellular iron content and growth temperature, was cloned into a multicopy-number plasmid. Physical mapping and complementation analysis established the position of cir between mgl and nfo on the Escherichia coli chromosome. A gene encoding a 32,000-dalton polypeptide was located downstream of and adjacent to cir, but did not appear to be part of the same transcriptional unit. A 525-base-pair fragment from the 5' end of the 1.8-kilobase-pair receptor-coding region directed iron-regulated transcription and translation of a hybrid cir-lacZ gene. Two overlapping promoters were identified by determination of the transcriptional start sites and by sequence analysis. A small open reading frame (120 nucleotides) of unknown significance preceded the receptor-coding sequence. Examination of the amino acid sequence of the receptor purified from the outer membrane revealed that the gene product was processed by removal of a signal peptide and that the mature form had an amino acid sequence near its amino terminus which closely resembled that of several other TonB-dependent proteins. |
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| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins/genetics; Base Sequence; Chromosome Mapping; Cloning, Molecular; Colicins/genetics; Cosmids; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation; Genes, Bacterial; Genetic Complementation Test; Iron/physiology; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Protein Biosynthesis; Protein Sorting Signals/genetics; Receptors, Cell Surface; Receptors, Immunologic/genetics; Temperature; Transcription, Genetic |
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