PMID:3308845
| Citation |
Sharma, RC and Smith, KC (1987) Role of DNA polymerase I in postreplication repair: a reexamination with Escherichia coli delta polA. J. Bacteriol. 169:4559-64 |
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| Abstract |
Using strains of Escherichia coli K-12 that are deleted for the polA gene, we have reexamined the role of DNA polymerase I (encoded by polA) in postreplication repair after UV irradiation. The polA deletion (in contrast to the polA1 mutation) made uvrA cells very sensitive to UV radiation; the UV radiation sensitivity of a uvrA delta polA strain was about the same as that of a uvrA recF strain, a strain known to be grossly deficient in postreplication repair. The delta polA mutation interacted synergistically with a recF mutation in UV radiation sensitization, suggesting that the polA gene functions in pathways of postreplication repair that are largely independent of the recF gene. When compared to a uvrA strain, a uvrA delta polA strain was deficient in the repair of DNA daughter strand gaps, but not as deficient as a uvrA recF strain. Introduction of the delta polA mutation into uvrA recF cells made them deficient in the repair of DNA double-strand breaks after UV irradiation. The UV radiation sensitivity of a uvrA polA546(Ts) strain (defective in the 5'----3' exonuclease of DNA polymerase I) determined at the restrictive temperature was very close to that of a uvrA delta polA strain. These results suggest a major role for the 5'----3' exonuclease activity of DNA polymerase I in postreplication repair, in the repair of both DNA daughter strand gaps and double-strand breaks. |
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| Keywords |
Chloramphenicol/pharmacology; DNA Polymerase I/metabolism; DNA Repair; DNA Replication; DNA, Bacterial/drug effects; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA, Bacterial/radiation effects; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/radiation effects; Genes, Bacterial; Mutation; Ultraviolet Rays |
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