PMID:3302609
| Citation |
Scholle, A, Vreemann, J, Blank, V, Nold, A, Boos, W and Manson, MD (1987) Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors. Mol. Gen. Genet. 208:247-53 |
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| Abstract |
The mglB gene of Escherichia coli codes for a galactose-binding protein (GBP) that serves both as the galactose chemoreceptor and as the recognition component of the beta-methylgalactoside transport system. The mglB551 mutation eliminates the chemotactic function of GBP without altering its transport or substrate-binding properties. To investigate the interaction between GBP and Trg, the chemotactic signal transducer for galactose, we sequenced the mglB genes from wild-type and mglB551 mutant strains. The mutation causes the replacement of Gly74 of GBP by Asp. This residue is located in alpha-Helix III at the tip of the P domain in the GBP tertiary structure farthest removed from the substrate-binding cleft between the P and Q domains. We conclude that Helix III must be part of, or at least adjacent to, the recognition site for Trg. Our sequence also included part of the mglA gene, which is immediately distal to mglB. The amino acid sequence deduced for the beginning of the MglA protein showed homology with a family of polypeptides that contain an ATP-binding site and are components of binding-protein-dependent transport systems. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins; Base Sequence; Biological Transport; Calcium-Binding Proteins; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chemoreceptor Cells/physiology; Chemotaxis; Escherichia coli/genetics; Escherichia coli/metabolism; Galactose/metabolism; Genes, Bacterial; Isoelectric Point; Macromolecular Substances; Monosaccharide Transport Proteins; Periplasmic Binding Proteins; Structure-Activity Relationship |
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