PMID:3298212
| Citation |
Kraus, W and Höltje, JV (1987) Two distinct transpeptidation reactions during murein synthesis in Escherichia coli. J. Bacteriol. 169:3099-103 |
|---|---|
| Abstract |
Murein synthesized in ether-permeabilized cells of Escherichia coli deficient in individual penicillin-binding proteins (PBPs) and in the presence of certain beta-lactam antibiotics was analyzed by high-pressure liquid chromatography separation of the muramidase split products. PBP 1b was found to to be the major murein synthesizing activity that was poorly compensated for by PBP 1a. A PBP 2 mutant as well as mecillinam-inhibited cells showed increased activity in the formation of oligomeric muropeptides as well as UDP-muramylpeptidyl-linked muropeptides, the reaction products of transpeptidation, bypassing the lipid intermediate. In contrast, penicillin G and furazlocillin severely inhibited these reactions but stimulated normal dimer production. It is concluded that two distinct transpeptidases exist in E. coli: one, highly sensitive to penicillin G and furazlocillin, catalyzes the formation of hyper-cross-linked muropeptides, and a second one, quite resistant to these antibiotics, synthesizes muropeptide dimers. |
| Links | |
| Keywords |
Amdinocillin/pharmacology; Azlocillin/analogs & derivatives; Azlocillin/pharmacology; Bacterial Proteins; Carrier Proteins/physiology; Cell Division; Escherichia coli/growth & development; Escherichia coli/metabolism; Hexosyltransferases; Imidazolidines; Muramoylpentapeptide Carboxypeptidase/physiology; Penicillin-Binding Proteins; Penicillins/pharmacology; Peptidoglycan/biosynthesis; Peptidyl Transferases/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.