PMID:3294808
| Citation |
Meganathan, R and Schrementi, J (1987) Tetrahydrothiophene 1-oxide as an electron acceptor for Escherichia coli. J. Bacteriol. 169:2862-5 |
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| Abstract |
Escherichia coli used tetrahydrothiophene 1-oxide (THTO) as an electron acceptor for anaerobic growth with glycerol as a carbon source; the THTO was reduced to tetrahydrothiophene. Cell extracts also reduced THTO to tetrahydrothiophene in the presence of a variety of electron donors. Chlorate-resistant (chl) mutants (chlA, chlB, chlD, and chlE) were unable to grow with THTO as the electron acceptor. However, growth and THTO reduction by the chlD mutant were restored by high concentrations of molybdate. Similarly, mutants of E. coli that are blocked in the menaquinone (vitamin K2) biosynthetic pathway, i.e., menB, menC, and menD mutants, did not grow with THTO as an electron acceptor. Growth and THTO reduction were restored in these mutants by the presence of appropriate intermediates of the vitamin K biosynthetic pathway. |
| Links | |
| Keywords |
Alcohol Dehydrogenase; Anaerobiosis; Cell Membrane/enzymology; Electron Transport; Escherichia coli/metabolism; Mutation; NAD/metabolism; NADP/metabolism; Oxidation-Reduction; Sulfoxides; Thiophenes/metabolism |
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