PMID:3285122

Citation	Condon, C and Weiner, JH (1988) Fumarate reductase of Escherichia coli: an investigation of function and assembly using in vivo complementation. Mol. Microbiol. 2:43-52
Abstract	Recombinant plasmids which carried portions of the Escherichia coli frd operon were constructed and their expression examined by in vivo complementation of E. coli MI1443. This strain lacked a chromosomal frd operon and was unable to grow anaerobically on glycerol and fumarate. Introduction of all four fumarate reductase subunits into E. coli MI1443 was essential for the restoration of growth. The FRD A, FRD B dimer (but neither subunit alone) was active in the benzyl viologen oxidase assay. Both FRD C and FRD D were required for membrane association of fumarate reductase and for the oxidation of reduced quinone analogues. Introduction into E. coli MI1443 of the frdABC and frdD genes on two separate plasmid vectors failed to restore anaerobic growth on glycerol and fumarate. Thus separation of the DNA coding for the FRD C and FRD D proteins affected the ability of fumarate reductase to assemble into a functional complex.
Links	PubMed
Keywords	Anaerobiosis; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Genes; Genes, Bacterial; Genetic Complementation Test; Kinetics; Multienzyme Complexes/genetics; Operon; Plasmids; Succinate Dehydrogenase/genetics
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