PMID:3284784
| Citation |
Riggs, PD, Derman, AI and Beckwith, J (1988) A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene. Genetics 118:571-9 |
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| Abstract |
It was shown previously that the secA gene of Escherichia coli is derepressed in cells that have a defect in protein export. Here it is demonstrated that the beta-galactosidase produced by a secA-lacZ gene fusion strain is regulated in the same way. Studies on the fusion strain reveal that the promoter or a site involved in regulation of the secA gene is located considerably upstream from the structural gene. The properties of the fusion strain provide a new selection for mutants that are defective in protein export. Selection for increased lac expression of a secA-lacZ fusion strain yields mutations in three of the known sec genes, secA, secD and prlA/secY. In addition, mutations in several genes not previously known to affect secA expression were obtained. A mutation in one of these genes causes a pleiotropic defect in protein export and a cold-sensitive growth defect; this gene, which maps at approximately 90 min on the bacterial chromosome, has been named secE. |
| Links | |
| Keywords |
Bacterial Proteins/secretion; Chromosome Mapping; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/physiology; Gene Expression Regulation; Genes, Bacterial; Lac Operon; Mutation |
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