PMID:3284654
| Citation |
Yamaguchi, K, Yu, F and Inouye, M (1988) A single amino acid determinant of the membrane localization of lipoproteins in E. coli. Cell 53:423-32 |
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| Abstract |
When beta-lactamase was fused with the signal peptide plus the amino-terminal 9 amino acid residues of the major outer membrane lipoprotein, the resultant lipo-beta-lactamase (LL-1) was shown to be localized to the outer membrane. However, when the 9 residue sequence was replaced with the amino-terminal 12 residue sequence of lipoprotein-28, an inner membrane protein, the resultant lipo-beta-lactamase (LL-2) was found exclusively in the inner membrane. The localization of LL-2 was shifted to the outer membrane simply by substituting the second amino acid residue (Asp) of LL-2 with Ser. Conversely, the alteration of the second residue (Ser) of LL-1 to Asp resulted in the localization of LL-1 to the inner membrane. These results suggest that the second amino acid residue of the lipoproteins plays a crucial role in determining their final locations in the E. coli envelope. |
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| Keywords |
Amino Acid Sequence; Cell Membrane/analysis; Centrifugation, Density Gradient; Electrophoresis, Polyacrylamide Gel; Escherichia coli/analysis; Escherichia coli/genetics; Escherichia coli/ultrastructure; Lipoproteins/analysis; Lipoproteins/genetics; Membrane Proteins/analysis; Membrane Proteins/genetics; Molecular Sequence Data; Mutation; Plasmids; Protein Sorting Signals/analysis; Protein Sorting Signals/genetics; Protein Sorting Signals/physiology; Recombinant Fusion Proteins/analysis; Recombinant Fusion Proteins/genetics; beta-Lactamases/analysis; beta-Lactamases/genetics |
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