PMID:3276667
| Citation |
Marek, LE and Henson, JM (1988) Cloning and expression of the Escherichia coli K-12 sad gene. J. Bacteriol. 170:991-4 |
|---|---|
| Abstract |
The Escherichia coli K-12 sad gene, which encodes an NAD-dependent succinic semialdehyde dehydrogenase, was cloned into a high-copy-number vector. Minicells carrying a sad+ plasmid produced a 55,000-dalton peptide, the probable sad gene product. |
| Links | |
| Keywords |
Bacterial Proteins/analysis; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Chromosome Mapping; Cloning, Molecular; Cosmids; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Hydroxybutyrate Dehydrogenase/biosynthesis; Hydroxybutyrate Dehydrogenase/genetics; Mutation; Nucleic Acid Hybridization; Plasmids; gamma-Aminobutyric Acid/analogs & derivatives; gamma-Aminobutyric Acid/metabolism |
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