PMID:3276659
| Citation |
Li, JM, Umanoff, H, Proenca, R, Russell, CS and Cosloy, SD (1988) Cloning of the Escherichia coli K-12 hemB gene. J. Bacteriol. 170:1021-5 |
|---|---|
| Abstract |
An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB. |
| Links | |
| Keywords |
Alleles; Chromosome Mapping; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Heme/biosynthesis; Heme/genetics; Hydroxymethylbilane Synthase/biosynthesis; Hydroxymethylbilane Synthase/genetics; Mutation; Plasmids; Porphobilinogen Synthase/biosynthesis; Porphobilinogen Synthase/genetics |
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