PMID:326762
| Citation |
Natter, W, Sens, D and James, E (1977) Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12. J. Bacteriol. 131:214-23 |
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| Abstract |
Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of the level of messenger RNA (mRNA) transcribed from seven of the nine genes of the arginine regulon of Escherichia coli K-12. The quantity of RNA complexing with each of the separated DNA strands of the argA, argF, argE, and argCBH operons carried on specialized transducing phages was measured. The derepressed:repressed ratio of mRNA formed in vivo was found to vary between about 3 and 4 when measured by hybridization to DNA isolated from specialized transducing phages carrying the argA, argE, argCBH, argF, and argI operons. |
| Links | |
| Keywords |
Arginine/biosynthesis; Coliphages; DNA, Bacterial; Escherichia coli/metabolism; Nucleic Acid Hybridization; Operon; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Transcription, Genetic; Transduction, Genetic |
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