PMID:3144039
| Citation |
Golden, JW and Wiest, DR (1988) Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene. Science 242:1421-3 |
|---|---|
| Abstract |
Two genome rearrangements involving 11- and 55-kilobase DNA elements occur during the terminal differentiation of an Anabaena photosynthetic vegetative cell into a nitrogen-fixing heterocyst. The xisA gene, located on the nifD 11-kilobase DNA element, was inactivated by recombination between the chromosome and a copy of the xisA gene that was mutated by inserting an antibiotic gene cassette. Site-directed inactivation of the Anabaena xisA gene blocked rearrangement of the 11-kilobase element and nitrogen fixation, but did not affect rearrangement of the 55-kilobase element, heterocyst differentiation, or heterocyst pattern formation. |
| Links | |
| Keywords |
Cyanobacteria/genetics; Cyanobacteria/growth & development; DNA/genetics; Gene Rearrangement; Genes; Genes, Bacterial; Immunoblotting; Nitrogen Fixation/genetics; Phenotype; Restriction Mapping |
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