PMID:3140549
| Citation |
Sirko, AE and Hulanicka, DM (1988) Cloning of cysE gene of Escherichia coli with a mini-Mu-lac containing a plasmid replicon. Acta Biochim. Pol. 35:51-5 |
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| Abstract |
The restriction map of cysE gene of Escherichia coli was established after cloning a mini-Mu-lac bacteriophage containing a plasmid replicon and recloning on pBR322 vector plasmid. Enzyme assays of transformants indicated the lack of autoregulation of cysE gene. |
| Links | |
| Keywords |
Acetyltransferases/genetics; Acetyltransferases/metabolism; Bacteriophages/genetics; Cloning, Molecular; Culture Media; DNA, Recombinant; Electrophoresis, Agar Gel; Escherichia coli/genetics; Genes, Bacterial; Genetic Vectors; Plasmids; Replicon; Restriction Mapping; Serine O-Acetyltransferase; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
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