PMID:3125860
| Citation |
Bezirdzhian, KhO, Kocharian, ShM and Akopian, ZhI (1987) [Hexamere purine nucleoside phosphorylase from Escherichia coli K-12. Kinetic analysis and mechanism of reaction]. Biokhimiia 52:1770-6 |
|---|---|
| Abstract |
A kinetic analysis of the phosphorolytic reaction catalyzed by hexameric purine nucleoside phosphorylase II from E. coli K-12 in the presence and absence of reaction products was carried out. The results of the kinetic analysis are consistent with a rapid equilibrium random Bi-Bi mechanism, in which a dead-end ternary (enzyme.purine base.phosphate) complex is formed. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Hypoxanthine; Hypoxanthines/pharmacology; Inosine/metabolism; Kinetics; Macromolecular Substances; Pentosyltransferases/isolation & purification; Phosphorylation; Protein Conformation; Purine-Nucleoside Phosphorylase/antagonists & inhibitors; Purine-Nucleoside Phosphorylase/isolation & purification; Purine-Nucleoside Phosphorylase/metabolism; Substrate Specificity |
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