PMID:3069589

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Citation

Kano, Y, Wada, M and Imamoto, F (1988) Genetic characterization of the gene hupA encoding the HU-2 protein of Escherichia coli. Gene 69:331-5

Abstract

The gene hupA encoding the HU-2 (HU-alpha) protein of Escherichia coli was mapped between rpoBC at 90 min and metA at 90.5 min on the K-12 genome by plasmid integration and P1-mediated transduction studies. Genetic studies using plasmid rescue techniques and physical mapping demonstrated that the gene order is rpoBC-hupA-rrnE-metA. The rpoBC is located approximately 10 kb proximal to the hupA gene, and rrnE about 8 kb distal to it. Thus, the direction of hupA gene transcription is clockwise on the E. coli map [Bachmann B.J., Microbiol. Rev. 47 (1983) 180-230].

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Keywords

Bacterial Proteins/genetics; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Genes; Genes, Bacterial; Genetic Complementation Test; Plasmids; Restriction Mapping; Transduction, Genetic

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