PMID:3065138
| Citation |
Mahan, MJ and Roth, JR (1988) Reciprocality of recombination events that rearrange the chromosome. Genetics 120:23-35 |
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| Abstract |
We describe a genetic system for studying the reciprocality of chromosomal recombination; all substrates and recombination functions involved are provided exclusively by the bacterial chromosome. The genetic system allows the recovery of both recombinant products from a single recombination event. The system was used to demonstrate the full reciprocality of three different types of recombination events: (1) intrachromosomal recombination between direct repeats, causing deletions; (2) intrachromosomal recombination between inverse homologies, causing inversion of a segment of the bacterial chromosome; and (3) circle to circle recombination (in the absence of any plasmid or phage functions). Results suggest that intrachromosomal recombination in bacteria is frequently fully reciprocal. |
| Links | |
| Keywords |
Chromosomes, Bacterial/physiology; Genes, Bacterial; Models, Genetic; Recombination, Genetic; Salmonella typhimurium/genetics; Transduction, Genetic |
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