PMID:3060037
| Citation |
Grogan, DW (1988) Temperature-sensitive murein synthesis in an Escherichia coli pdx mutant and the role of alanine racemase. Arch. Microbiol. 150:363-7 |
|---|---|
| Abstract |
The basis for disruption of morphogenesis by depletion of pyridoxine derivatives was studied using a pdxH null mutant of Escherichia coli K-12. Removal of pyridoxal from growing cultures severely inhibited murein synthesis in vivo, whereas simultaneous supplementation with D-alanine effectively prevented inhibition. Extractable alanine racemase was low following such starvation. Selection of mutants overcoming the glycine- or temperature-sensitivity imposed by pyridoxine limitation yielded a variety of phenotypes. The most effective of these extragenic suppressors conferred an elevated alanine racemase activity which was resistant to the effects of pyridoxal removal. |
| Links | |
| Keywords |
Alanine/metabolism; Alanine Racemase/metabolism; Amino Acid Isomerases/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Mutation; Peptidoglycan/biosynthesis; Pyridoxal/metabolism; Pyridoxine/metabolism; Suppression, Genetic; Temperature |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.