PMID:3058704
Citation |
Rolfes, RJ and Zalkin, H (1988) Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. Cloning, nucleotide sequence, and interaction with the purF operator. J. Biol. Chem. 263:19653-61 |
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Abstract |
The Escherichia coli gene purR, encoding a repressor protein, was cloned by complementation of a purR mutation. Gene purR on a multicopy plasmid repressed expression of purF and purF-lacZ and reduced the growth rate of host cells by limiting the rate of de novo purine nucleotide synthesis. The level of a 1.3-kilobase purR mRNA was higher in cells grown with excess adenine, suggesting that synthesis of the repressor may be regulated. The chromosomal locus of purR was mapped to coordinate 1755-kb on the E. coli restriction map (Kohara, Y., Akiyama, K., and Isono, K. (1987) Cell 50, 495-508). Pur repressor bound specifically to purF operator DNA as determined by gel retardation and DNase I footprinting assays. The amino acid sequence of Pur repressor was derived from the nucleotide sequence. Pur repressor subunit contains 341 amino acids and has a calculated Mr of 38,179. Pur repressor is 31-35% identical with the galR and cytR repressors and 26% identical with the lacI repressor. These four repressors are likely homologous. Amino acid sequence similarity is greatest in an amino-terminal region presumed to contain a DNA-binding domain. A similarity is also noted in the operator sites for these repressors. |
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Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Genes; Genes, Bacterial; Genetic Complementation Test; Genotype; Molecular Sequence Data; Operon; Plasmids; Purine Nucleotides/biosynthesis; Repressor Proteins/genetics; Restriction Mapping; Sequence Homology, Nucleic Acid; Transcription Factors/genetics |
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