PMID:3041371
| Citation |
Zhang, JR and Deutscher, MP (1988) Escherichia coli RNase D: sequencing of the rnd structural gene and purification of the overexpressed protein. Nucleic Acids Res. 16:6265-78 |
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| Abstract |
We have determined the nucleotide sequence of a 1.4-kb-pair fragment of the E. coli chromosome that carries the complete rnd gene encoding RNase D, a putative tRNA processing enzyme. The coding region of rnd extends for a total of 1128 nucleotides beginning at an initiator UUG codon and terminating at a UAA codon, and encodes a 375-amino acid polypeptide of 42,679 daltons, consistent with the known size of RNase D. A rapid purification procedure was developed for isolation of RNase D from strains overexpressing the enzyme. The N-terminal sequence and the amino acid composition of the homogenous protein were in excellent agreement with those derived from the sequence of the rnd gene. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Codon; Endoribonucleases/genetics; Endoribonucleases/isolation & purification; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation; Genes, Bacterial; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; Protein Biosynthesis; Ribonuclease III |
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