PMID:3040683

Citation	Wang, MD, Liu, L, Wang, BM and Berg, CM (1987) Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene. J. Bacteriol. 169:4228-34
Abstract	avtA, which encodes the alanine-valine transaminase, transaminase C, was cloned in vivo with high- and low-copy-number mini-Mu cloning vectors. The phenotype conferred by the cloned avtA+ gene usually depended upon the plasmid copy number; most high-copy-number avtA+ plasmids permitted isoleucine-requiring ilvE strains to grow in the absence of isoleucine (multicopy suppression), while low-copy-number avtA+ plasmids did not. avtA was mapped to a 1.25-kilobase segment by comparison of the restriction maps of 24 independent mini-Mu plasmids and then by gamma-delta (Tn1000) mutagenesis of a pBR322-avtA+ plasmid. The direction of transcription of avtA on the cloned fragment was determined with fusions to a promoterless lac gene.
Links	PubMed PMC213734
Keywords	Chromosome Mapping; Cloning, Molecular; DNA Restriction Enzymes; DNA Transposable Elements; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Mutation; Phenotype; Plasmids; Transaminases/biosynthesis; Transaminases/genetics; Transcription, Genetic; Transduction, Genetic
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