PMID:3037486
| Citation |
Rostas, K, Morton, SJ, Picksley, SM and Lloyd, RG (1987) Nucleotide sequence and LexA regulation of the Escherichia coli recN gene. Nucleic Acids Res. 15:5041-9 |
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| Abstract |
The nucleotide sequence of a 2224 bp region of the Escherichia coli chromosome that carries the LexA regulated recN gene has been determined. A region of 1701 nucleotides encoding a polypeptide of 567 amino acids with a predicted molecular weight of 63,599 was identified as the most probable sequence for the recN structural gene. The proposed initiation codon is preceded by a reasonable Shine-Dalgarno sequence and a promoter region containing two 16 bp sequences, separated by 6 bp, that match the consensus sequence (SOS box) for binding LexA protein. DNA fragments containing this putative promoter region are shown to bind LexA in vitro and to have LexA-regulated promoter activity in vivo. The amino acid sequence of RecN predicted from the DNA contains a region that is homologous to highly conserved sequences found in several DNA repair enzymes and other proteins that bind ATP. A sequence of 9 amino acids was found to be homologous to a region of the RecA protein of E. coli postulated to have a role in DNA/nucleotide binding. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Chromosomes, Bacterial/physiology; DNA Repair; DNA Restriction Enzymes; Escherichia coli/genetics; Genes; Genes, Bacterial; Repressor Proteins/metabolism; Sequence Homology, Nucleic Acid; Serine Endopeptidases; Transcription Factors/metabolism |
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