PMID:3033442
| Citation |
Akiyama, M, Horiuchi, T and Sekiguchi, M (1987) Molecular cloning and nucleotide sequence of the mutT mutator of Escherichia coli that causes A:T to C:G transversion. Mol. Gen. Genet. 206:9-16 |
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| Abstract |
The Escherichia coli mutator gene mutT, which causes A:T----C:G transversion, was cloned in pBR 322. mutT+ plasmids carry a 0.9 kb PvuII DNA fragment derived from the E. coli chromosome. Specific labelling of plasmid-encoded proteins by the maxicell method revealed that mutT codes for a polypeptide of about 15,000 daltons. The protein was overproduced when the mutT gene was placed under the control of the lac regulatory region on a multicopy runaway plasmid. The nucleotide sequence of the mutT gene was determined by the dideoxy method. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Base Composition; Base Sequence; Cloning, Molecular; DNA Restriction Enzymes; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Genetic Vectors; Genotype; Mutation; Phosphoric Monoester Hydrolases; Plasmids; Pyrophosphatases |
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