PMID:3031020

Citation	Thoms, B and Wackernagel, W (1987) Regulatory role of recF in the SOS response of Escherichia coli: impaired induction of SOS genes by UV irradiation and nalidixic acid in a recF mutant. J. Bacteriol. 169:1731-6
Abstract	We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF, recN, and sulA, by UV irradiation and nalidixic acid was examined. Induction of eight genes by the two agents was impaired by recF400::Tn5 to different extents. The ninth fused SOS gene, dinF, was no longer inducible by UV when combined with recF400::Tn5. The generally impaired SOS response in recF strains did not result from weak induction of recA protein synthesis, since a recA operator-constitutive mutation did not alleviate the inhibitory effect of the recF mutation. The results suggest that recF plays a regulatory role in the SOS response. It is proposed that this role is to optimize the signal usage by recA protein to become a protease.
Links	PubMed PMC212006
Keywords	Bacterial Proteins/genetics; DNA Repair; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Mutation; Nalidixic Acid/pharmacology; Operon; Recombination, Genetic; SOS Response (Genetics); Ultraviolet Rays
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