PMID:3029692
| Citation |
Adachi, T, Mizuuchi, M, Robinson, EA, Appella, E, O'Dea, MH, Gellert, M and Mizuuchi, K (1987) DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy. Nucleic Acids Res. 15:771-84 |
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| Abstract |
We have determined the sequence of the E. coli gyrB gene, using a new sequencing approach in which transposition from a mini-Mu plasmid into the DNA provides random start points for dideoxynucleotide sequence analysis. The gyrB sequence corresponds to a protein 804 amino acids long; a previously isolated protein fragment with partial enzymatic activity has been identified as the C-terminal half-molecule. A plausible terminator of gyrB transcription is located just beyond the structural gene. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; DNA Restriction Enzymes; Escherichia coli/genetics; Genes; Genes, Bacterial; Plasmids |
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