PMID:3015722
| Citation |
Ismailov, ZF, Smirnov, SP and Tarasov, VA (1986) [Characteristics of the het mutations in the Escherichia coli chromosome causing an increase in Tn1 transposition frequency]. Genetika 22:777-86 |
|---|---|
| Abstract |
Four mutations were studied which lead to increasing the frequency of transposon Tn1 translocation into different replicons. These mutations (het1, het2, het3 and het4) increase the frequency of Tn1 translocation 10-20-fold. The het1 mutation is recessive and has been localized in the 90-94.5 min region of the bacterial chromosome. The mutation effects Tn1 transposition in the presence of F plasmid only. As we have demonstrated recently, F-plasmid inhibits Tn1 transposition in Escherichia coli cells. The het1 mutation eliminates this inhibition. Unlike het2, het3 and het4 mutations, het1 is responsible for resistance to male phages f1, f2, MS2 and inhibition of conjugative transfer in F+ bacteria. |
| Links | |
| Keywords |
Chromosome Mapping; Chromosomes, Bacterial/ultrastructure; DNA Transposable Elements; Escherichia coli/genetics; F Factor; Genes, Bacterial; Genes, Recessive; Lac Operon; Mutation; Phenotype; Plasmids; Replicon |
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