PMID:3012538
| Citation |
Windle, BE and Hays, JB (1986) A phage P1 function that stimulates homologous recombination of the Escherichia coli chromosome. Proc. Natl. Acad. Sci. U.S.A. 83:3885-9 |
|---|---|
| Abstract |
Recombination between two different defective lacZ genes in the Escherichia coli chromosome (lac- X lac- recombination) was stimulated 2- to 8-fold by prophage P1, depending on the nature of the phage c1 repressor. The P1 BamHI restriction fragment B8 in a lambda-P1:B8 hybrid phage, stimulated lac- X lac- recombination 90-fold in the absence of P1 repressor. A gene necessary for recombination enhancement, designated ref, was localized to one end of B8. Ref expression from lambda-P1:B8 was repressed in trans by a P1 c+ prophage. Two P1 regulatory mutations, bof and lxc, derepressed prophage expression of ref and depressed a prophage function that complemented an E. coli mutant (ssb) deficient in the single-stranded DNA binding protein. Ref stimulation was dependent on preexisting E. coli recombination functions (RecA-RecBC and RecA-RecF). However, other (phage and plasmid) recombination processes involving these functions were not stimulated. ref::Tn5 phages plated and formed lysogens normally. Thus ref appears to be an integral, but not essential, phage gene that stimulates recombination of the host chromosome specifically. |
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| Keywords |
Bacterial Proteins/genetics; Chromosome Mapping; Chromosomes, Bacterial/physiology; Coliphages/genetics; DNA, Bacterial/genetics; DNA, Viral/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases/genetics; Gene Expression Regulation; Genes, Viral; Genetic Complementation Test; Lysogeny; Rec A Recombinases/genetics; Recombination, Genetic; Virus Replication |
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