PMID:3012467
| Citation |
Burns, DM and Beacham, IR (1986) Nucleotide sequence and transcriptional analysis of the E. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5'-nucleotidase): regulation of the ushA gene, and the signal sequence of its encoded protein product. Nucleic Acids Res. 14:4325-42 |
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| Abstract |
The DNA sequence of the ushA gene, encoding UDP-sugar hydrolase (5'-nucleotidase), has been determined. The amino-terminal sequence encodes a signal peptide whose predicted processing site is confirmed by N-terminal amino acid analysis of purified mature UshA protein. The signal sequence contains a concentration of rare codons in comparison with the mature sequence. The origins of transcription from the ushA promoter have been determined, using primer extension. Three transcripts, originating within a 6 bp region, were identified and might be related to three overlapping potential -10 hexamers in the ushA promoter region. There was a discernable change in the relative proportion of these transcripts during growth-phase regulation of the ushA gene. |
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| Keywords |
5'-Nucleotidase; Amino Acid Sequence; Base Sequence; DNA Restriction Enzymes/metabolism; DNA, Bacterial/analysis; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Nucleotidases/genetics; Promoter Regions, Genetic; Protein Sorting Signals/analysis; Protein Sorting Signals/genetics; Transcription, Genetic |
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