PMID:3006041
| Citation |
Masai, H, Bond, MW and Arai, K (1986) Cloning of the Escherichia coli gene for primosomal protein i: the relationship to dnaT, essential for chromosomal DNA replication. Proc. Natl. Acad. Sci. U.S.A. 83:1256-60 |
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| Abstract |
The Escherichia coli gene encoding one of the primosomal proteins, protein i, was cloned by the use of synthetic oligonucleotide probes. Nucleotide sequence analysis revealed a coding region for protein i of 537 base pairs preceded by a possible promoter sequence. The gene is located adjacent to the dnaC locus, probably both being in a single operon. The protein i gene was shown to be closely related to the dnaT locus based on the following observations. (i) A multicopy plasmid carrying only the protein i gene suppresses the temperature-sensitive phenotype of a dnaT strain and restores the ability of the strain to carry out stable DNA replication in the absence of protein synthesis. (ii) An extract from a dnaT strain does not support replication of the plasmid pBR322 in vitro; addition of purified protein i restores its activity. These results indicate that protein i is encoded by dnaT and that it is essential for chromosomal DNA replication and is involved in the induction of stable DNA replication during the SOS response. |
| Links | |
| Keywords |
Bacterial Proteins/genetics; Cloning, Molecular; DNA Replication; Escherichia coli/genetics; Gene Expression Regulation; Genes; Genes, Bacterial; Genetic Linkage; Plasmids |
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