PMID:3001024

Citation	DeFeyter, RC and Pittard, J (1986) Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12. J. Bacteriol. 165:226-32
Abstract	The gene aroL in Escherichia coli K-12, specifying shikimate kinase II, was contransduced with proC at a frequency of 99%. The gene order is lac proC aroL. A 2.7-kilobase BamHI fragment containing aroL+ was cloned into pBR322. This plasmid conferred highly elevated levels of shikimate kinase synthesis which were subject to repression control by tyrR. The aroL gene was localized within a 730-base-pair region by both subcloning and insertional mutagenesis with Tn1000. A second gene, designated aroM and encoding a protein of molecular weight 26,000, is cotranscribed with aroL. Transcription proceeds in the order aroL aroM in a clockwise direction on the chromosome. The function of aroM remains unknown.
Links	PubMed PMC214393
Keywords	Bacterial Proteins/genetics; Bacterial Proteins/physiology; Chromosome Mapping; Cloning, Molecular; DNA Transposable Elements; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Operon; Phosphotransferases/genetics; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Transcription, Genetic; Transduction, Genetic
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