PMID:3000890

Citation	Rushlow, KE, Deutch, AH and Smith, CJ (1985) Identification of a mutation that relieves gamma-glutamyl kinase from allosteric feedback inhibition by proline. Gene 39:109-12
Abstract	A 1.75-kb DNA fragment containing the entire Escherichia coli proB+ gene has been sequenced. The proB locus encodes the structural gene for gamma-glutamyl kinase (GK), the enzyme responsible for the first step in proline biosynthesis, and the primary regulatory point of the pathway. We have previously reported the nucleotide (nt) sequence of a mutant proB gene isolated from an E. coli strain resistant to the toxic analog of proline, 3,4-dehydro-DL-proline (DHP). This mutant gene encodes a GK which is refractory to allosteric feedback inhibition by proline (DHPR). Comparison of the proB+ and DHPR proB sequences revealed a single base difference, an A-T to C-G transversion localized at nt position 428 within the amino acid (aa) coding region of proB. This mutation predicts an aa change from glutamic acid in the wild-type (wt) enzyme to alanine in the DHPR enzyme.
Links	PubMed
Keywords	Allosteric Regulation/drug effects; Base Sequence; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Mutation; Phosphotransferases/antagonists & inhibitors; Phosphotransferases/genetics; Phosphotransferases (Carboxyl Group Acceptor); Proline/pharmacology
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