PMID:2999087
| Citation |
Ishiguro, N and Sato, G (1985) Nucleotide sequence of the gene determining plasmid-mediated citrate utilization. J. Bacteriol. 164:977-82 |
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| Abstract |
The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrophobic polypeptide and which is preceded by a reasonable ribosomal binding site. However, the single polypeptide found in minicell experiments had an apparent molecular weight of 35,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Deletion; Citrates/metabolism; Citric Acid; Cloning, Molecular; DNA Restriction Enzymes/metabolism; DNA Transposable Elements; Escherichia coli/genetics; Fluorometry; Nucleic Acid Conformation; Phenotype; Plasmids |
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