PMID:2997135
| Citation |
Kawamukai, M, Kishimoto, J, Utsumi, R, Himeno, M, Komano, T and Aiba, H (1985) Negative regulation of adenylate cyclase gene (cya) expression by cyclic AMP-cyclic AMP receptor protein in Escherichia coli: studies with cya-lac protein and operon fusion plasmids. J. Bacteriol. 164:872-7 |
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| Abstract |
We constructed cya-lac protein and operon fusion plasmids in vitro. The effect of cyclic AMP (cAMP) on cya expression was examined by measuring the synthesis of beta-galactosidase in Escherichia coli cells containing fused plasmids. In the cya-lacZ fused protein system, cya expression was strongly repressed by exogenous cAMP. Functional cAMP receptor protein (CRP) was necessary for this effect. On the other hand, in a tet-lacZ fused protein as a control system, tet expression was not affected by cAMP. The inhibition of cya expression by cAMP was also observed in the cya-lac fused operon system, although it was necessary to increase the amount of cAMP or CRP in the cells to detect the effect. The results indicate that cAMP-CRP is a negative regulator of cya expression at the level of transcription. |
| Links | |
| Keywords |
Adenylate Cyclase/biosynthesis; Adenylate Cyclase/genetics; Cyclic AMP/pharmacology; DNA, Recombinant; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation/drug effects; Genes, Bacterial; Operon; Plasmids; Receptors, Cyclic AMP/pharmacology; Receptors, Cyclic AMP/physiology; Transcription, Genetic; beta-Galactosidase/biosynthesis |
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