PMID:2989252
| Citation |
Asada, K, Nakatani, S and Takanami, M (1985) Cloning of the contiguous 165-kilobase-pair region around the terminus of Escherichia coli K-12 DNA replication. J. Bacteriol. 163:398-400 |
|---|---|
| Abstract |
Escherichia coli K-12 chromosomal DNA was partially digested with either EcoRI or HindIII, and cosmid libraries were constructed. By screening these libraries, a series of partially overlapping clones which covered the terC region was isolated. The cloned area spanned about 165 kilobase pairs, corresponding to the 29.7-to-33.2-min region of the genetic map of the E. coli chromosome. |
| Links | |
| Keywords |
Chromosome Mapping; Chromosomes, Bacterial/ultrastructure; Cloning, Molecular; DNA Replication; DNA Restriction Enzymes/diagnostic use; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial |
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