PMID:2988449
| Citation |
Chu, J, Shoeman, R, Hart, J, Coleman, T, Mazaitis, A, Kelker, N, Brot, N and Weissbach, H (1985) Cloning and expression of the metE gene in Escherichia coli. Arch. Biochem. Biophys. 239:467-74 |
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| Abstract |
A lambda-transducing phage was isolated that contains the metE gene. This gene codes for N5-methyl-H4-folate:homocysteine methyltransferase (EC 2.1.1.14), an enzyme that catalyzes the terminal reaction in methionine biosynthesis. A 9.1-kb EcoR1 fragment of this phage, containing the metE gene, was then cloned into pBR325. This plasmid, pJ19, was used to transform Escherichia coli strain 2276, a metE mutant, and restore the MetE+ phenotype. Although the transformed cells produced large amounts of the metE protein in vivo, in vitro studies using pJ19 as template showed low synthesis of the metE protein. |
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| Keywords |
Bacteriophage lambda/genetics; Centrifugation, Density Gradient; Cloning, Molecular; DNA Restriction Enzymes/metabolism; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Methyltransferases/genetics; Microscopy, Electron; Phenotype; Plasmids; Transduction, Genetic |
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