PMID:2963943
| Citation |
Hollifield, WC, Kaplan, EN and Huang, HV (1987) Efficient RecABC-dependent, homologous recombination between coliphage lambda and plasmids requires a phage ninR region gene. Mol. Gen. Genet. 210:248-55 |
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| Abstract |
A phage lambda gene that gives a 100-fold increase in recombinant frequencies for RecABC pathway-mediated, phage-plasmid homologous recombination (Shen and Huang 1986) maps to ninG (orf 204) of lambda. We call this gene rap, for recombination adept with plasmid. A similar determinant exists in Charon 4A and maps in phi 80-derived sequences, between nin5 and the Rz homology with lambda. The absence of the Rap+ phenotype from certain lambda vectors explains the inefficiency of screening the resulting phage libraries using phage-plasmid homologous recombination. The mapping of rap permits the construction of lambda vectors more suitable for this screening technique. |
| Links | |
| Keywords |
Bacteriophage lambda/genetics; Chromosome Mapping; Escherichia coli/genetics; Exodeoxyribonuclease V; Exodeoxyribonucleases/genetics; Genes, Viral; Plasmids; Rec A Recombinases/genetics; Recombination, Genetic |
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