PMID:2958600
| Citation |
Plaha, DS and Rogers, HJ (1987) Effect of osmotic shock and shearing forces on ferric enterochelin transport in Escherichia coli K12. J. Gen. Microbiol. 133:1227-34 |
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| Abstract |
The fes mutation in Escherichia coli K12, which inactivates enterochelin esterase, allows the cell to accumulate ferric enterochelin. The ferric complex of enterochelin was released in significant quantities from a fes mutant after osmotic shock. Analysis of the effects of the individual stages of the shock procedure in wild-type cells showed that prior exposure of cells to sucrose and EDTA was not required, careful dilution of cells into a hypo-osmolar medium being sufficient to induce efflux of Fe3+. Prior treatment with EDTA or exposure to shearing forces served either to enhance efflux or to induce efflux in isotonic media. Neither vitamin B12 nor 5'-nucleotidase was released from the periplasm by these procedures. The release observed under mild conditions was stimulated specifically by Co2+, did not occur at 0 degree C, and was inhibited by 2,4-dinitrophenol at 37 degrees C. From these observations, it was concluded that the efflux of Fe3+ represents a physiological response of the cell to exposure to a hypo-osmolar medium. Such changes may enhance survival following physicochemical stressing of the bacterial outer membrane. |
| Links | |
| Keywords |
Biological Transport; Cations/metabolism; Enterobactin/metabolism; Escherichia coli/metabolism; Iron Radioisotopes/diagnostic use; Osmotic Pressure; Radioisotope Dilution Technique; Serine/analogs & derivatives |
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