PMID:2892489
| Citation |
Suzuki, H, Kumagai, H, Echigo, T and Tochikura, T (1988) Molecular cloning of Escherichia coli K-12 ggt and rapid isolation of gamma-glutamyltranspeptidase. Biochem. Biophys. Res. Commun. 150:33-8 |
|---|---|
| Abstract |
Based on the results of mapping of ggt, eight strains were selected from a gene library of E. coli. One of the strains harboring pLC9-12 was found to show 14 times higher gamma-glutamyltranspeptidase activity per cell than the wild type strain. The ggt was subcloned to the BamHI site of pUC18 and the recombinant plasmid pSH101 was obtained. Ggt- phenotype of gamma-glutamyltranspeptidase-deficient mutants was complemented by pSH101. The specific activity of the enzyme in cells harboring pSH101 was 37-fold higher than that in the wild type cells. gamma-Glutamyltranspeptidase was isolated from the periplasmic fraction of the cells by simple two steps and crystallized. |
| Links | |
| Keywords |
Cloning, Molecular; DNA Restriction Enzymes; DNA, Bacterial/genetics; DNA, Recombinant; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Molecular Weight; Plasmids; Transformation, Bacterial; gamma-Glutamyltransferase/biosynthesis; gamma-Glutamyltransferase/genetics; gamma-Glutamyltransferase/isolation & purification |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.