Wang, MD, Buckley, L and Berg, CM (1987) Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids. J. Bacteriol. 169:5610-4
To facilitate molecular analyses of a previously uncharacterized gene involved in alanine synthesis, attempts were made to clone the wild-type allele of this gene, alaA, with a mini-Mu plasmid element used for in vivo cloning. Seventy-six independent Ala+ plasmids were isolated and characterized. Physiological, enzymological, and restriction endonuclease analyses indicated that three different genes, none of them alaA, were cloned. These genes were avtA+, which encodes the alanine-valine transaminase (transaminase C); tyrB+, which encodes the tyrosine-repressible transaminase (transaminase D); and a previously undescribed gene, called alaB, which encodes an alanine-glutamate transaminase.
Alanine/biosynthesis; Alanine/genetics; Alanine Transaminase/genetics; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Isoleucine/genetics; Plasmids; Transaminases/genetics; Transduction, Genetic; Tyrosine Transaminase/genetics
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