From EcoliWiki
Jump to: navigation, search

Wang, MD, Buckley, L and Berg, CM (1987) Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids. J. Bacteriol. 169:5610-4


To facilitate molecular analyses of a previously uncharacterized gene involved in alanine synthesis, attempts were made to clone the wild-type allele of this gene, alaA, with a mini-Mu plasmid element used for in vivo cloning. Seventy-six independent Ala+ plasmids were isolated and characterized. Physiological, enzymological, and restriction endonuclease analyses indicated that three different genes, none of them alaA, were cloned. These genes were avtA+, which encodes the alanine-valine transaminase (transaminase C); tyrB+, which encodes the tyrosine-repressible transaminase (transaminase D); and a previously undescribed gene, called alaB, which encodes an alanine-glutamate transaminase.


PubMed PMC214003


Alanine/biosynthesis; Alanine/genetics; Alanine Transaminase/genetics; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Isoleucine/genetics; Plasmids; Transaminases/genetics; Transduction, Genetic; Tyrosine Transaminase/genetics


You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.



EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also


See Help:References for how to manage references in EcoliWiki.