PMID:2883171
| Citation |
Jenkins, LS and Nunn, WD (1987) Regulation of the ato operon by the atoC gene in Escherichia coli. J. Bacteriol. 169:2096-102 |
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| Abstract |
The expression of the Ato enzymes, acetyl coenzyme A:acetoacetyl coenzyme A transferase and thiolase II, is required for growth of Escherichia coli on short-chain fatty acids. The structural genes for these enzymes, atoD, atoA, and atoB, respectively, make up the ato operon. A 48-kilodalton protein encoded by atoC was required for the synthesis or activation of the Ato enzymes. The expression of Ato enzyme activities was inducible in atoC+ strains, constitutive in atoCc strains, and noninducible in atoC mutants. Merodiploid studies demonstrated that the atoCc allele is trans-dominant to the atoC+ allele. To study the action of the trans-acting atoC-encoded activator, the promoter of the ato operon was fused to the promoterless galK gene and introduced into a low-copy-number vector. The resulting low-copy-number fusion plasmid was introduced into atoC+, atoC, and atoCc hosts. The expression of the fused galK gene was inducible in the atoC+ host, noninducible in atoC host strains, and constitutive when harbored in the atoCc host. This indicated that the atoC+ and atoCc gene products act at the level of transcription, stimulating the expression of the ato operon. A working model consistent with these results is presented. |
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| Keywords |
Acetoacetates/metabolism; Acetyl-CoA C-Acetyltransferase/genetics; Butyrates/metabolism; Cloning, Molecular; Coenzyme A-Transferases; DNA-Binding Proteins/genetics; Enzyme Induction; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Operon; Promoter Regions, Genetic; Sulfurtransferases/genetics |
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