PMID:2861200

Citation	Porter, AC, Kumamoto, C, Aldape, K and Simoni, RD (1985) Role of the b subunit of the Escherichia coli proton-translocating ATPase. A mutagenic analysis. J. Biol. Chem. 260:8182-7
Abstract	A plasmid that carries the uncF gene of Escherichia coli, which codes for the b subunit of the proton-translocating ATPase (F1F0), was mutagenized with hydroxylamine and ethyl methanesulfonate. Mutated plasmids were characterized by complementation analysis and by in vitro transcription/translation. Eleven of the plasmids mutated specifically in uncF were studied in detail: the nucleotide sequences of their uncF genes were determined and their effects on the F1F0 were measured. The results suggest that a C-terminal portion of the b subunit that involves the glycine residue at position 131, is required for the formation of a functional proton pore as well as for the binding of F1 to F0. A mutation in the N-terminal portion of the b subunit, in the glycine residue at position 9, also prevented the formation of a functional proton pore, but had only a small effect on the binding of F1 to F0.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Macromolecular Substances; Plasmids; Proton-Translocating ATPases/genetics; Proton-Translocating ATPases/metabolism; Spectrometry, Fluorescence
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