PMID:2841565

Citation	Pon, CL, Calogero, RA and Gualerzi, CO (1988) Identification, cloning, nucleotide sequence and chromosomal map location of hns, the structural gene for Escherichia coli DNA-binding protein H-NS. Mol. Gen. Genet. 212:199-202
Abstract	Beginning with a synthetic oligonucleotide probe derived from its amino acid sequence, we have identified, cloned and sequenced the hns gene encoding H-NS, an abundant Escherichia coli 15 kDa DNA-binding protein with a possible histone-like function. The amino acid sequence of the protein deduced from the nucleotide sequence is in full agreement with that determined for H-NS. By comparison of the restriction map of the cloned gene and of its neighboring regions with the physical map of E. coli K12 as well as by hybridization of the hns gene with restriction fragments derived from the total chromosome, we have located the hns gene oriented counterclockwise at 6.1 min on the E. coli chromosome, just before an IS30 insertion element.
Links	PubMed
Keywords	Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial/physiology; Cloning, Molecular; DNA Restriction Enzymes; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Genes; Genes, Bacterial; Molecular Sequence Data; Nucleotide Mapping
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